Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Pseudoalteromonas haloplanktis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 43% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
Ca2+ | 52% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
Co2+ | 36% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
Cu2+ | 57% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
EDTA | 47% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
Fe3+ | complete inhibition at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
Hg2+ | 11% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
Mg2+ | 54% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
Mn2+ | 55% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
phenylmethylsulfonyl fluoride | complete inhibition at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
SDS | 0.9% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme is associated to the outer membrane | Pseudoalteromonas haloplanktis | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas haloplanktis | Q3IF07 | - |
- |
Pseudoalteromonas haloplanktis TAC 125 | Q3IF07 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Q-Sepharose column chromatography | Pseudoalteromonas haloplanktis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.079 | - |
enzyme from solubilised medium, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis |
0.95 | - |
after 12fold purification, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Pseudoalteromonas haloplanktis TAC 125 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl caproate + H2O | best substrate | Pseudoalteromonas haloplanktis | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Pseudoalteromonas haloplanktis TAC 125 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Pseudoalteromonas haloplanktis TAC 125 | 4-nitrophenol + pentanoate | - |
? | |
tricaprylin + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
tricaprylin + H2O | - |
Pseudoalteromonas haloplanktis TAC 125 | ? | - |
? | |
triolein + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
triolein + H2O | - |
Pseudoalteromonas haloplanktis TAC 125 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lip1 | - |
Pseudoalteromonas haloplanktis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
at pH 7.1 | Pseudoalteromonas haloplanktis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 50 | at pH 7.1 | Pseudoalteromonas haloplanktis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 40 | activity increases up to 40°C | Pseudoalteromonas haloplanktis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
reaches the apparent maximal activity at pH values above 8.5 | Pseudoalteromonas haloplanktis |